The Association of pre-mir-196a2 T/C Polymorphism and Risk of Gastric Cancer in Ardabil, Iran

Micro-RNAs are a large group of non-coding RNAs of 18-25 nucleotides in length. Polymorphisms in the human pre-mir-RNAs could change the efficiency of target cell and can also play a role in cell cycle regulation and cancer. The aim of this study is to investigate the association of rs11614913 T/C polymorphism with gastric cancer risk in Ardabil Province. In a case-control study, this polymorphism was evaluated by PCR-RFLP method. For this purpose, the genomic DNA was extracted from blood samples of 50 subjects with gastric cancer and 50 healthy controls. The 149-bp amplified fragments in PCR were treated with MspI. In tumor samples, the frequencies of CC, TC, and TT genotypes were 14%, 40%, and 46%, respectively whereas these ratios were 8%, 28%, and 64%, respectively. The frequency of CC genotype in case group was somewhat different from the control group (14% vs. 8%), But this difference was not statistically significant (p-value = 0. 18). In this study, we did not find any significant association between rs11614913 T/C polymorphism and gastric cancer risk in Ardabil Province.